To examine the role of HIF-1α in induction of osteoclastogenic factors in neuroblastoma cells, we established neuroblastoma SH-SY5Y cells stably transfected with pCI-neo expression vector containing HIF-1α cDNA or pGSH1-GFP vector containing HIF-1α shRNA sequence or luciferase shRNA sequence. Here, HIF1A is linked to neuroblastoma.