PPARA and metabolic dysfunction-associated steatotic liver disease: Concordantly, we observed that PPARα-KO mice had increases in the expression of ACC mRNA with consequently exacerbated ectopic deposition of fat in the liver, whereas WT mice had lower expression of ACC, which indicated a balancing regulatory mechanism in these animals, most likely via the activation of PPARα; this may explain why WT mice showed lower NAFLD and inflammation in response to the HFD.