To investigate in depth the small RNA production during different adenovirus infections, we sequenced the cytoplasmic (referred to as Cyto) and RISC bound (referred to as RISC-IP) small RNA pools isolated at 24 hpi from Ad4, Ad5, Ad11, and Ad37-infected 293-Flag-Ago2 cells. This evidence concerns the gene AGO2 and adenoviridae infectious disease.