To verify the tumor initiating capacity of the HFB grown cells, we injected 5 immune deficient nude mice/group a range of 1×10∧2, 1×10∧3, 1×10∧4, and 1×10∧5 cells grown in the HFB (∼96% CD133+) and compared their growth to an equal number of CD133(+) MACsorted cells and CD133(−) cells for 3 months. Here, PROM1 is linked to neoplasm.