Ten proteins (CEA, p53, HER2, NY-ESO-1, Hsp60, Hsp70, MYCL1, CHEK2, HNRNPK, and NME1) were immobilized onto microstructured glass slides functionalized with six different surface chemistries to detect autoantibodies in sera of breast cancer patients. Here, ERBB2 is linked to breast carcinoma.