The top nine candidates in the screen (Fig1B, Supplementary Fig S1)—showing the highest repressed levels of normalized TCF-luficerase reporter activity above the baseline given by dnTCF—were then selected and tested for inhibition of human Ls174T colon cancer cell proliferation, as this is WNT-TCF-dependent (van de Weteringet al, 2002). The gene discussed is HNF4A; the disease is malignant colon neoplasm.