As in Ls174T cells, 4B5 treatment modified TCF targets and cell proliferation in primary human local colon cancer TNM4 CC14 cells (without detected mutations inAPC orβ-CATENIN) and in primary colon cancer liver metastasis mCC11 cells (Varnatet al, 2009) in a dose-dependent manner (Supplementary Fig S2). This evidence concerns the gene HNF4A and malignant colon neoplasm.