HNF4A and colonic neoplasm: To further investigate the specificity of Ivermectin and Selamectin for the WNT-TCF pathway, we attempted to rescue their effects in colon cancer cells by directly enhancing TCF function via TCFVP16, a fusion of the TCF DNA-binding domain plus the strong VP16 viral transcriptional transactivator (Kimet al, 2000) that acts in a WNT- and β-CATENIN-independent manner (Supplementary Fig S6).