Pretreatment with Z-VAD-FMK resulted in total blocking of 10058-F4 induced caspase-3 activity and a significant decrease in the inhibition of 10058-F4-mediated proliferation in both cells (Figure 3I and J), suggesting that inducing mitochondrial apoptosis may be a major mechanism to inhibit cell proliferation in 10058-F4 treated ovarian cancer cells. This evidence concerns the gene CASP3 and ovarian carcinoma.