Further, we established an miRNA-panel to diagnose NAFLD and revalidated the panel in a large number of serum samples.Moreover, we compared the AUC of the miRNA panel with those of ALT,miRNA-122 and FIB-4,the miRNA panel is superior to seven other non-invasive markers in NAFLD Patients. The gene discussed is GPT; the disease is metabolic dysfunction-associated steatotic liver disease.