MLKL and alkaline phosphatase measurement: RIP3 can also directly phosphorylate MLKL to cause the translocation of MLKL to the plasma membrane and subsequent membrane rupture and necrosis [172–174]. RIP3 or MLKL KO mice are resistant to cerulein-induced AP, suggesting that necroptosis plays a critical role in animal experimental AP, but its relevance to human AP is not clear.