HIF1A and breast cancer: Treatment for 24 hr to 150 μM CoCl2 alone had little or no effect on total levels of Akt, PI3K, and mTOR or phospho-Akt but did cause a relatively large increase in phospho-mTOR, HIF-1α, phospho-p70S6K, phospho-elF-4E1 levels in +SA mammary tumor cells, as compared to the vehicle-treated control group (Figure 5).