IGFBP1 and hydrops fetalis: Hepatic IGF1 and IGFBP1 gene expression was significantly higher in HF fetuses when compared to the C-HF (p < 0.001) and HF-C group (p < 0.0001) (Figure 2).Exposure to a HF diet during the second half of pregnancy (C-HF) significantly increased the hepatic gene expression of IGF1 (p < 0.01), IGF1R (p < 0.01) and insulin-like growth factor 2 (IGF2) (p < 0.01) when compared to the C group.