CDH1 and neoplasm: Previous studies reported that MDA-MB-231 cells failed to form spheroids and lacked E-cadherin expression when grown on semi-solid methylcellulose [7], [39]–[41], or in round bottom culture well coated with a poly-HEMA [42].These findings suggest that extracellular matrix, such as collagen fragments in Col-Tgel, are important for tumor spheroid formation.