To determine whether our approach could be extended to other histotypes and cell surface markers, we also conducted spiking experiments with the breast cancer cell line MCF-7 (isolated based on HER2 labeling), colon cancer line SW480 (EpCAM), and the non-small cell lung cancer line H292 (EGFR) (experimental parameters listed in Table 1). The gene discussed is EPCAM; the disease is breast cancer.