DNA promoter methylation of the tumor suppressor genes was one of the key mechanism for gene silence.The aim of this study is to investigate the difference of MGMT gene promoter methylation rate in tumor tissue and autologous controls (serum, normal lung tissue and bronchial lavage fluid) in patients with non-small cell lung cancer (NSCLC). The gene discussed is MGMT; the disease is non-small cell lung carcinoma.