We found that retroviral interference of Twist 1 expression induced the expression of PPARγ during differentiation induction and increased the secretion of multiple adipokines into the medium; however, interference of Twist 1 expression did not significantly impair the process of lipid formation, indicating a potential role for Twist 1 in obesity and associated diseases. Here, PPARG is linked to obesity due to melanocortin 4 receptor deficiency.