In the current study, we demonstrate that upregulation of miR-27a is critical for cisplatin resistance and tumor metastasis of lung adenocarcinoma cells both in vitro and in vivo, and miR-27a induces mesenchymal features and promotes tumor metastasis of chemoresistant lung adenocarcinoma via silencing RKIP. Here, PEBP1 is linked to lung adenocarcinoma.