MoM kisspeptin was significantly higher in twin pregnancies unaffected by miscarriage when compared with singleton pregnancies unaffected by miscarriage (MoM kisspeptin: 1.06 ± 0.42, singleton, no miscarriage, 1.64 ± 0.54, twin no miscarriage, P < .001, vs singleton, no miscarriage; 2.09 ± 0.26, triplet, no miscarriage, P < .05, vs singleton, no miscarriage) (data not shown). This evidence concerns the gene KISS1 and spontaneous abortion.