Figure 1I shows that the kinetics of WNV-induced CPE follows a linear trend and correlates well with the multiplicity of infection for each serial dilution (R2 = 0.968). The onset of WNV-induced CPE was independent of TLR3 status as determined by ANOVA comparison of the MOI dependence fit curves (Figure 1G–I). Here, TLR3 is linked to infection.