Overall, high levels of methylation of the GAS7 (prognosis cut-off value = 52.62%; high-methylation: 43.3%, low-methylation: 56.7%; χ2 p = 0.03) and ALX4 genes (prognosis cut-off value = 25.8%; high-methylation: 67.1%, low-methylation: 32.9%; χ2 p = 0.005) associated with poor DFS in CRC patients (data not shown). The gene discussed is ALX4; the disease is colorectal carcinoma.