IGFBP3 and colorectal carcinoma: The mean methylation levels for each gene in primary CRC tissues were: 23.8% for SEPT9, 50.5% for TWIST1, 44.9% for IGFBP3, 50.2% for GAS7, 36.5% for ALX4, and 35.3% for miR137. As shown in Figure 1, methylation levels of each gene were significantly higher in CRC versus normal mucosa from healthy subjects (p<0.0001 to p<0.0005 for all comparisons).