TWIST1 and colorectal carcinoma: Accordingly, each gene was classified as methylated when mean methylation levels were higher than 7.1% for SEPT9, 35.7% for TWIST, 27.5% for IGFBP3, 53.5% for GAS7, 28.5% for ALX4, and 11.9% for miR137. Based upon these analyses, the most frequently methylated markers in CRC tissues were the miR-137 gene (302/344, 87.7%), followed by IGFBP3 (289/348, 83%), TWIST1 (269/356, 75.6%), SEPT9 (244/346, 70.5%), ALX4 (214/350, 61.1%), and GAS7 (164/378, 43.3%).