To further substantiate confocal results, we investigated the interaction of the NPs and mycobacteria at the subcellular level in parallel through immunoisolation of the endosomes using protein paramagnetic beads conjugated to Rab5, Rab 7, Rab 11, and Rab 14, following drug treatment or infection with M. smegmatis. This evidence concerns the gene RAB11A and infection.