The authors enrolled 34,346 subjects who completed a health check-up, and excluded subjects with risk factors associated with elevated ALT levels, including high body mass index, high waist circumference, high glucose levels, high cholesterol levels, low levels of HDL, high levels of TG, hepatitis B virus surface antigen, anti-hepatitis C virus antibody and NAFLD. The gene discussed is GPT; the disease is metabolic dysfunction-associated steatotic liver disease.