TNF and early-onset autosomal dominant Alzheimer disease: Indeed, in clarifying and confirming the biological effects of rs4986790 TLR4 polymorphism and its role in the pathophysiology of age-related diseases, including CVDs, Alzheimer disease, prostate cancer, diabetes, and longevity, we assessed the levels of IL-6, TNF-α, IL-10, and eicosanoids in LPS-stimulated whole blood samples in vitro of 50 young healthy Sicilians, screened for the presence of rs4986790 TLR4 and −765G>C PTGS2, −1708G>A 5-Lo polymorphisms [54].