Although the differences in metabolic functions of PFKFB3 and PFKFB4 are poorly understood, we observed a more robust induction of PFKFB4 protein relative upon exposure of H460 cells to 1% oxygen and significant correlation between hypoxia and PFKFB4 expression in lung adenocarcinoma tissues. This evidence concerns the gene PFKFB3 and lung adenocarcinoma.