The importance of PFKFB4 as a potential target for the development of cancer therapeutics was then significantly expanded in 2012 by two independent groups that conducted unbiased screens for genes essential for cancer survival and found that PFKFB4 is required for both glioma stem-like cell [14] and prostate cancer cell survival [15] but not for normal cell survival. This evidence concerns the gene PFKFB4 and central nervous system cancer.