A difference between liver and spleen pathology is seen when comparing the typhoid and colitis models: while the typhoid fever model is characterized by marked hepatic necrosis, this was not regularly observed in the colitis model, while marked hepatic inflammatory cell influx is seen in both models (Figure 5J-L) Moreover, in the colitis model Nlrp3−/− (and to a lesser extent, Asc−/− mice) displayed slightly increased spleen and liver parenchyma inflammation with corresponding elevated AST, albeit not ALT, levels when compared to WT mice. Here, PYCARD is linked to colitis.