In contrast, PBLs from 19 out of 22 MCAD patients demonstrated marked increase in SP-triggered PGD2 release revealing a significantly (P < 0.001) enhanced mean PGD2 level of 1896 ± 389.7 pg/ml (range: 939 to 2303 pg/ml), as compared to 130 ± 53.5 pg/ml (range: 50–294 pg/ml) for healthy individuals (Figures 4 and 5). This evidence concerns the gene PTGDS and medium chain acyl-CoA dehydrogenase deficiency.