[Notably, mice inoculated with Flt3L-secreting B16 melanoma cells also exhibited a pronounced increase in size of the hepatic DC population consisting of immature cDCs and pDCs (data not shown).] Subsequently, the purified splenic DC population was transfected by electroporation with NTC8685-eRNA41H-EGFP (GFP-expressing plasmid) in order to determine transfection efficiency. Here, FLT3LG is linked to melanoma.