In general, samples with low TIN-estimates (≤ − 1.0) were separated from samples with high TIN-estimates (≥1.0), although not perfectly in colorectal cancer series II and prostate cancer series I. This association between TIN-estimates and expression levels of splicing factors was less clear in the other three cancer datasets (gastric cancer, lung cancer series I, and neuroblastoma; Figure 1). This evidence concerns the gene SLU7 and lung cancer.