In this study mode of cell death or determination of apoptosis event induced by goniothalamin on bladder cancer cell line RT4 was assessed by using Annexin V-FITC/PI Labeling assay as described previously [25], at the concentration of 50, 61 (IC50) &100 μM of goniothalamin following 24 hr treatment. This evidence concerns the gene ANXA5 and urinary bladder carcinoma.