At 24 h after the infection of eMDM with EIAVFDDV13, IFNβ expression was up-regulated by 10- to 20-fold at the mRNA level and 4- to 6-fold at the protein level compared with the mock-treated control; the timing of this up-regulation was correlated with the temporal up-regulation of TLR3 expression in eMDM infected with EIAVFDDV13. This evidence concerns the gene IFNB1 and infection.