Considering the correlation between strong induction of infection resistance and elevated TLR3 and IFNβ expression induced by attenuated EIAVFDDV13 as well as the existence of a signaling pathway linking TLR3 activation with IFNβ expression, the effect of TLR3 activation on infection resistance induced by EIAVFDDV13 was mimicked by treating eMDM with poly I:C, a TLR3 ligand. This evidence concerns the gene IFNB1 and infection.