To investigate the potential anticancer activity of hinokitiol on human lung adenocarcinoma cells, six different human lung adenocarcinoma cell lines with different EGFR status, A549 (EGFRwt), PC9 (EGFRdel19), H1299 (EGFRwt), H3255 (EGFRL858R), PC9-IR (EGFRdel19, with resistance to gefitinib) and H1975 (EGFRL858R+T790M, with resistance to gefitinib) cells, were treated with hinokitiol (5 and 10 μM) for 48 and 72 h. Here, EGFR is linked to lung adenocarcinoma.