To test whether TSC inhibits growth of different types of breast cancer, we treated two immortalized mammary epithelial cell lines (MCF10A and 184B5), two ERα positive breast cancer cell lines (MCF7 and T47D), and two ERα negative breast cancer cell lines (SW527 and HCC1937) at varying concentrations of TSC (0.125, 0.25, 0.5, 1, 2 mg/ml) for 48 hours and then measured the cell viability via the SRB assay. Here, ESR1 is linked to breast cancer.