Indirect ELISA for calculating antibodies level for whole M. leprae sonicated antigen (WMLS) in serum samples where we have found serological positivity of 10/22 (45.45%), 9/35 (25.71%), and 15/32 (46.88%) in leprosy cases with anti-IgA, IgG, and IgM was taken as detector antibody in leprosy patients, respectively. This evidence concerns the gene CD40LG and leprosy.