CD40LG and leprosy: Indirect ELISA for calculating antibodies level for whole M. leprae sonicated antigen (WMLS) in serum samples where OD values have cut-off values (0.206, 0.5, and 0.325) (Figure 2) at dilution 1 : 1600, 1 : 400, and 1 : 800 of serum, respectively, with anti-IgA, IgG, and IgM was taken as detector antibody in untreated leprosy patients, respectively.