Joanna et al. found that TLR9 is expressed in human prostate cancer cell lines LnCaP, C4-2B, Du-145, PC3, and in clinical samples of prostate cancer through immunohistochemistry and western blotting, but not in MDA Pca2b and stromal cells of the clinical adenocarcinoma samples (32). This evidence concerns the gene TLR9 and Familial prostate cancer.