In this study, we have demonstrated that: 1) the CYP-induced model of cystitis showed features of storage symptoms, 2) gap junction formation was up-regulated in the bladder smooth muscle layer of the model mice, 3) spontaneous contraction was enhanced in detrusor smooth muscles taken from the treated mice, 4) gap junction blockade alleviated storage symptoms in the model mice. The gene discussed is PPIG; the disease is cystitis.