In addition, we found the expression of HPV16/18 DNA and p53 protein in patients withaxillary lymph node metastasis is significantly higher than those without, and the expression of p53 protein increase with TMN staging advancing, which demonstrate that the expression of HPV16/18 DNA and p53 protein may correlate closely with the prognosis of breast cancer. The gene discussed is TP53; the disease is breast cancer.