We detected the express of HPV16/18 DNA and p53 protein in 85 in-patients in the department of breast surgery of our hospital from September 2005 to October 2011, the purpose of our study was 1) To compare the expression of HPV 16/18 DNA and p53 protein in invasive ductal carcinoma, breast fibroadenoma and normal breast speciman; 2) To determine the correlation between HPV 16/18 infection and p53 gene in breast cancer to facilitate the clinical diagnosis. The gene discussed is TP53; the disease is invasive ductal breast carcinoma.