Patients with upregulated DPYSL3 had a significantly higher prevalence of lymph node metastasis, overall distant metastasis and peritoneal dissemination, indicating that DPYSL3 is a metastasis facilitator of GC, and high expression of DPYSL3 may predict the metastatic behavior associated with an invasive GC phenotype. This evidence concerns the gene DPYSL3 and metastatic malignant neoplasm in the lymph nodes.