A set of proteins that was found to be up-regulated (RNASE7, S100A7A, KRT4, ELANE, and ECM1) or inconsistently up-regulated (S100A7) in cholesteatoma in the large-scale analysis was also found to be significantly up-regulated (p<0.05) in the SRM analyses. The gene discussed is S100A7; the disease is cholesteatoma.