Mann-Whitney U significance data confirmed that l-myc-2 (p = 0.03) was the only antigen to significantly differentiate between cancer and healthy serum cohorts (HCC > healthy controls), and AFP-C (p = 0.04), NY-ESO-1 (p = 0.04) and DKK1 (p = <0.01) were the only antigens to significantly differentiate between cancer and high-risk serum cohorts (HCC > high-risk controls). Here, DKK1 is linked to hepatocellular carcinoma.