NAE1 and Familial prostate cancer: As shown in Figure 1(a), NEDD8-activating enzyme E1 (NAE1 and UBA3), NEDD8-conjugating enzyme E2 (UBC12 and UBE2F), and NEDD8-E3 ligases (DCN-1 and ROC1) were expressed in high levels, suggesting the activation of neddylation pathway in prostate cancer cells.