AKT1 and colorectal carcinoma: In addition, downstream kinases PI3K, Src homology 2 domain containing (Shc), protein kinase B (AKT), and extracellular signal-regulated kinase (ERK) were determined using the multiplexed immunomicroarray based Collaborative Enzyme Enhanced Reactive (CEER) immunoassay [17]–[19] in 120 CRC patients from stage I to IV that included 116 primary, 15 liver metastasis, and 1 peritoneal seeding tissue samples.