In this study, our protocol was shown to mimic prototypical characteristics of clinical atopic asthma, namely blood, tissue and BALF eosinophilia, an elevation of TH2 cytokines IL-5 and IL-13 in BALF, and prominent AHR, as shown by a heightened reactivity to methacholine. This evidence concerns the gene IL13 and Increased total eosinophil count.