Accordingly, we used Ang II-stimulated cultured cardiomyocytes and mice with transverse aortic constriction (TAC) to examine the following issues: (1) the influence of Ankrd1/CARP on cardiac hypertrophy; (2) whether Ankrd1/CARP modulates calcineurin and nuclear factor of activated T cells (NFAT) and (3) whether the ARB olmesartan medoxomil prevents the upregulation of Ankrd1/CARP in response to Ang II or pressure overload. Here, AGT is linked to cardiac hypertrophy.