EGFR and neoplasm: Another cause of these differences is most likely technical issues related to genotyping and the sensitivity of particular molecular methods may influence the effectiveness of EGFR. Direct sequencing technique requires more than 50 % of tumor cells in the specimen (e.g., surgical material), whereas real-time PCR and next-generation sequencing techniques (CE-IVD methods) allow EGFR detection in samples containing as few as 1 % tumor cells.