The SCID-rab mouse model was used for the in vivo study, with two rabbit bones contralaterally implanted in each SCID mouse; one of the two bones was injected with INA6 cells infected with enhanced green fluorescent protein/luciferase (for tracking cells and monitoring tumor burden) and with shRNA (scrambled control or BTK-KD). The gene discussed is AGFG1; the disease is neoplasm.