AGFG1 and Miyoshi myopathy: BTK protein was markedly reduced in INA6 MM cells stably infected with lentiviral particles containing BTK shRNA (hereafter BTK-KD cells), as assessed by western blots (Supplementary Figure S1A); BTK gene expression was 96% lower in BTK-KD cells than in cells infected with control scrambled shRNA, and expression remained low after cells were engrafted in the SCID-rab mouse model for MM (Supplementary Figures S1B and C).