MYOM2 and infection: To ensure that the detected (−)-strand RNA was specifically from reporter-containing BM25A or sBM25A rather than from a recombinant molecule, RT-PCR using primers that anneal to the reporter sequence in BM25A or sBM25A (for RT) and the M protein gene in the BCoV genome was performed to test for a potential recombinant molecule generated during infection [14].