FACS analysis demonstrated that the pre-addition of rapamycin rather than CQ to A549/PTX cells significantly increased the number of apoptotic cells upon NDV/FMW infection compared with NDV/FMW infection alone (Figure 3C and 3D, *p < 0.05; **p < 0.01), supporting by the observation that treatment with rapamycin but not CQ enhanced the cleavage of caspase 3 in NDV/FMW-infected A549/PTX cells compared with virus alone (Figure 3A). This evidence concerns the gene CASP3 and infection.