We ascertained that the pre-treatment with 10 ng/mL TNF-α for 2 hours induced insulin resistance in both vascular cells as shown by the significant decreases in the phosphorylation of Akt, p70S6K and p44/42 in cells stimulated with 10 nmol/L insulin for 10 minutes (Figure 1B). This evidence concerns the gene RPS6KB1 and Insulin resistance.