HNRNPD and infection: Figure 4 shows immunofluorescent staining of AUF1 in EV71-infected RD cells; DAPI was used to stain nuclei. While AUF1 was predominantly nuclear 4 h post-infection, it was distributed evenly in the nucleus and cytoplasm 6 h post-infection, and was almost exclusively in the cytoplasm 8 h post-infection. As expected, infected cells expressed the EV71 3A protein in the cytoplasm (Fig. 4).