In the clinic, it has been used to image diseases like Jadassohn–Pellizzari anetoderma,119 scleroderma,120 lymphedema,121 atopic dermatitis in a mouse model,122 and actinic keratosis.123 In some of these diseases, it is often the fact that both collagen and elastin can be easily resolved by SHG and two-photon autofluorescence, respectively, that makes multiphoton imaging particularly suitable. This evidence concerns the gene ELN and scleroderma.