In this context it has been demonstrated that palmitate-induced IR in C2C12 myotubes is associated with impaired expression of myokines (i.e. irisin, CTRP15 [family with sequence similarity 132, member B] and FGF21 [fibroblast growth factor 21]) known for their potential beneficial roles in metabolic diseases [27]. This evidence concerns the gene ERFE and metabolic disease.